Limitations of Chromatography FAQ What is chromatography ? What are the different types of chromatography ? What is the principle behind chromatography ? What are the applications of chromatography ? What is the difference between HPLC and GC? How does chromatography separate components in a mixture? What is the role of the stationary phase in ... Chromatography is a process for separating components of a mixture. To get the process started, the mixture is dissolved in a substance called the mobile phase, which carries it through a second substance called the stationary phase. The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another. The nature of the specific mobile and stationary phases determines which substances travel more quickly or slowly, and is ... Thin Layer Chromatography (TLC) Thin layer chromatography (TLC) is a laboratory technique used to separate and identify the components of a mixture. It is similar to paper chromatography , but it uses a thin layer of a solid adsorbent material, such as silica gel or alumina, coated onto a glass plate or plastic sheet as the stationary phase. Chromatography Definition Chromatography is a method of separating the constituents of a solution, based on one or more of its chemical properties. This could be charge, polarity, or a combination of these traits and pH balance. In essence, the solution is passed through a medium which will hinder the movement of some particles more than others. This draws the different molecules apart as they travel through the medium. Often, different dyes are used to represent the different parts, or ...