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ELISA stands for enzyme-linked immunosorbent assay , also often referred to as enzyme immunoassay (EIA). ELISA is a type of immunological assay used to detect and quantify specific biomolecules, such as proteins, antigens, or antibodies, in biological samples. ELISA stands for Enzyme-Linked Immunosorbent Assay . ELISA is widely used to detect diseases such as HIV/AIDS, hepatitis, dengue fever, and COVID-19. The Enzyme-Linked Immunosorbent Assay (ELISA) operates on the concept of antigen-antibody binding. The enzyme-linked immunosorbent assay ( ELISA ) (/ ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] . What is ELISA? The Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used laboratory method designed to identify and measure specific proteins, antibodies, antigens, or hormones in biological samples.