Gram staining is one of the most crucial staining techniques in microbiology. The name comes from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882 to identify organisms causing pneumonia.[1] Typically, Gram staining is the first test performed, utilizing crystal violet or methylene blue as the primary color.[2] Organisms that retain the primary color and appear purple-brown under a microscope are gram-positive. In contrast, those that do not take up the primary ... Learn how to differentiate gram positive and gram negative bacteria using crystal violet, iodine, safranin and alcohol. See the animation, examples and reagents used in gram staining technique. Gram stain is a technique to classify bacteria into gram -positive and gram -negative based on their cell wall properties. Learn how it works, who invented it and when it is used in medicine and research. Gram stain is the most widely used standard procedure in microbiology that is used to classify bacteria according to their cell wall composition. It distinguishes the bacteria into two major groups; Gram -positive and Gram -negative bacteria. see : Difference between gram -positive and gram -negative bacteria. It is a valuable diagnostic tool in both research and clinical settings. It helps in the characterization and identification of bacteria.
