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High-performance liquid chromatography or commonly known as HPLC , is an analytical technique used to separate, identify or quantify each component in a mixture. What is HPLC (High-performance liquid chromatography)? High-Performance Liquid Chromatography ( HPLC ) is a sophisticated analytical technique used extensively in various scientific fields to separate, identify, and quantify the components of a mixture. This technique, originally known as high-pressure liquid chromatography, has evolved significantly since its inception. HPLC operates on the principle of column chromatography but utilizes advanced technology to enhance its performance. Unlike ... HPLC is a technique for separating and detecting compounds dissolved in a liquid sample using a column and a detector. Learn about the HPLC apparatus, separation, and chromatogram in this web page. HPLC is an analytical chemistry technique applied to separate, identify, and quantify semi- and non-volatile compounds in liquid samples. Industries like pharma and biopharma, environmental, and food and beverage use HPLC for the characterization, purification, and quantitation of compounds.