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STERILIZATION meaning: 1. the process of having a medical operation to make it impossible to have children: 2. the…. Learn more. Sterilization definition: the act of sterilizing.. See examples of STERILIZATION used in a sentence. The meaning of STERILIZATION is the act or process of sterilizing. How to use sterilization in a sentence. Sterilization describes a process that destroys or eliminates all forms of microbial life and is carried out in health-care facilities by physical or chemical methods. Steam under pressure, dry heat, EtO gas, hydrogen peroxide gas plasma, and liquid chemicals are the principal sterilizing agents used in health-care facilities.